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CONTEXT
phosphate buffer (50 mM Na HPO2 4, 0.3 M NaCl, pH 7.4), bound protein was eluted upon addition of elution buffer (50 mM Na HPO2 4, 0.3 M NaCl, 20 mM EDTA, pH 7.0). Green fluorescent fractions were then collected [...] Fig. 7 and Table 2, approximately 60 % of ZZGFPuv was secreted into the medium upon induction with the temperature shift while less than 10% was secreted under the normal growth condition.
Figure 7: Im [...] Journal 2005;4:91-104 – ISSN 1611-2156 Received: 22. September 2005, accepted 4. October 2005, published: 7. October 2005
Original article:
Construction of chimeric antibody binding green fluorescent protein …